Sero-molecular epidemiology of hepatitis E virus in pigs and human contacts in Ghana
One Health Outlook volume 3, Article number: 13 (2021)
Hepatitis E virus (HEV) is among the leading causes of viral hepatitis in most developing countries. Zoonotic acquisition of HEV genotype 3 from swine has come into focus more recently. Available studies on HEV in Ghana and other countries in the region do not provide enough information towards understanding the epidemiology of HEV in human and animal populations. Towards this end, we conducted a comparative cross-sectional study to determine the seroprevalence and risk factors associated with HEV exposure, both in swine and humans working on pig farms in typical local settings. The presence of viral RNA in human and swine samples was also evaluated, along with classification of viral sequences from HEV-positive samples.
Structured questionnaires soliciting information on pigs reared, as well as socio-demographic information including age, sex and educational background of humans was collected. A total of 10 ml and 5 ml of whole blood was collected from pigs and human participants respectively. ELISA and real-time RT-PCR were performed on the sera for the qualitative detection of IgG antibodies to hepatitis E virus and viral RNA, respectively.
Five hundred and forty-four (544) human participants including 264 swine contacts and 280 swine non-contacts were enrolled in the study. Although the proportion of HEV IgG antibodies was higher in contact groups (114; 54.3%) than non-contact groups (96; 45.7%), a multivariate analysis did not show any significant difference. No HEV RNA was detected in human samples. Similarly, 720 pigs were sampled from 18 farms located in five regions in Ghana. Twenty-three (23) of the pigs (3.2, 95%CI = 2.0–4.8) were positive for HEV RNA by real-time RT-PCR testing. Sequences obtained from HEV-positive samples were found to share high sequence identities with each other and clustered with other genotype 3 viruses indicating the existence of circulating zoonotic genotype 3 viruses on farms. Although we did not find evidence of pig to human transmission of HEV genotype 3, the presence of this genotype in pigs shows the potential for possible zoonotic transmission in African farm settings and buttresses the importance of active surveillance for the infection among at risk populations.
Hepatitis E virus (HEV) is the leading cause of viral hepatitis in much of the developing world . Even though the infection has a worldwide distribution, the highest prevalence rates are observed in developing countries particularly in East and South Asia and Africa [2,3,4,5,6,7]. Annually, an estimated 20 million HEV infections occur worldwide leading to approximately 56,600 deaths . HEV infection is usually self-limiting, but may develop into fulminant hepatitis with case-fatality rate rising from 2% in the general population to 40% among pregnant women . The virus has at least 4 different types: genotypes 1, 2, 3 and 4. Genotypes 1 and 2 have been found only in humans . Genotypes 3 and 4 circulate in several animals (including pigs, wild boars, and deer) without causing overt disease, and occasionally infect humans . HEV is spread mainly by the faecal-oral route via contaminated water  and ingestion of undercooked meat or meat products derived from infected animals (e.g. pork liver) . Of note, the zoonotic nature of HEV genotype 3 acquired from swine has been grossly underestimated in Europe. Not only does the virus cause persistent infection with a risk to develop liver cirrhosis in immunosuppressed patients ; recent studies and growing clinical experience show that the virus not only causes hepatitis, but an array of mainly peripheral neurological syndromes [14, 15].
Pigs are the most important reservoir for HEV genotype 3 . However, in pigs, HEV in most cases causes only subclinical infection [17, 18]. This notwithstanding, the risk of zoonotic transmission to humans is an important public health concern  and warrants a search for risk factors of infection.
Better appreciation of genotype 3 during the last 10–15 years has triggered re-adjustments of serological test antigens and PCR primers . Only with these improvements has it become clear that HEV prevalence in European populations is high in the range of 5% or even above [21,22,23,24,25,26,27,28,29,30]. There have been studies on HEV in Ghana [31,32,33,34,35,36,37,38,39,40], but these have not targeted genotype 3 specifically. Studies focusing on zoonotic hepatitis E in sub-Saharan Africa should therefore aim to detect genotype 3 and investigate sources of infection (swine) along with humans. Because important information on the influence of animal contact on infection cannot easily be derived from mere prevalence data, studies should try to link infections via sequencing and ask for rates and conditions of animal contact. As the geographic diversity in African countries is huge, they should cover a larger geographic space as well as places of different levels of infrastructure and development.
In the present study we employed both serological and molecular techniques to assess the prevalence of HEV in humans and swine, investigate HEV genotypes and determine risk of infection associated with working or living in close proximity to pig farms.
Study areas, design, and sampling strategy
The research was a comparative cross-sectional study that was conducted as part of a prospective investigation of possible zoonotic agents in livestock and wildlife in Ghana. The study was conducted between June, 2015 and May, 2016 in communities having pig farms in five Regions in Ghana. The Regions were the Ashanti, Greater Accra, Northern, Upper East and Volta Region (Fig. 1).
Study design for pig sampling
Prior to commencement of the study, a sample size of at least 124 pigs was calculated using an expected blood HEV RNA of 2.1%  and a confidence level of 95%. The formula used for detecting at least one positive animal was computed from Annon and Roe .
Where n is the required samples size; N is the average herd size; d is the expected number of infected animals and p is the confidence level of 95%.
The farms were selected using a simple two-stage cluster sampling technique. At the first level of sampling, Regional Veterinary Officers at all the ten regions of Ghana were contacted for information on swine populations in their respective regions. A comprehensive list of farms with swine populations was obtained and regions having farms with cumulative herd size of ≥50 pigs were selected. Each of the farms selected in the region were further examined and a subset of farms were selected taking into consideration accessibility to the farms and willingness of farm managers to have their pigs sampled. For each farm we counted the number of animals and selected representative animals based on proportion based sample size. Where particular pig pens have huge numbers, we selected every second pig until a pre-determined sample size was reached.
Each of the farms selected was visited by the research team. During these visits, the reason for the study, how the study was to be conducted and information on use of data was provided to the farm managers. Farm managers were also allowed to ask questions and were further encouraged to seek clarification on issues they were concerned about. The farms of managers who consented to the study were enrolled and every individual pig was assessed and sampled. In order to minimize possible injury to the animals, piglets less than 2 months old and heavily pregnant sows were excluded from the study. Variables assessed on pigs included number of pigs reared by the respondents, estimated age, gender and body scoring. Veterinarians assisted in measuring clinical parameters such as presence of fever, neurological disorders, diarrhoea, icterus and other signs of disease. Fever status was assessed based on rectal temperatures above 40 °C degree Celsius .
Study design for human sampling
A sample size of 86 each for contact and non-contact groups was determined using an expected seroprevalence of 15% among contact groups and 2% among non-contact groups , a study power of 80 and confidence level of 95%. For the purpose of this study, we defined “contacts” as individuals who directly take care of the pigs and those who live together with pigs in their households. “Non-contacts” were those living in the same community/vicinity with the contact groups but not involved in taking care of pigs or living together with the pigs in their household. All contacts and non-contacts were approached and those who consented were enrolled and sampled. All respondents were interviewed with a questionnaire which had been pre-tested and revised. Variables collected from human contacts and non-contacts included age, gender, religion, occupation, highest level of education, availability of toilet facility and consumption of pork. We also asked participants about the source of their drinking water. Questionnaires were designed such that participants could select a “yes” or “No” option for each type of drinking water source.
A minimum of 5 and 10 ml of blood was collected from humans and pigs, respectively. All samples were transported via cold chain to the Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR) where testing was carried out. Molecular analysis was performed on both pig and human samples. Additional serological analysis was performed on only human samples.
Serological analysis of human samples
Samples were tested for the presence of IgG antibodies to HEV. The test was performed using an Enzyme-Linked Immunosorbent Assay (ELISA) for in-vitro qualitative detection of IgG antibodies to hepatitis E virus in human serum according to manufacturer’s (Axiom Diagnostics, Germany) instructions. This kit has sensitivity and specificity of about 100 and 99% respectively [45,46,47,48]. Briefly 100 μl of sample diluent was first added to micro-plates already pre-coated with recombinant antigens corresponding to the structural regions of HEV Open Reading Frame-2. Ten microliters (10 μl) of study samples including negative and positive controls were added to each pre-labelled sample wells. The plates were incubated at 37 °C and 100 μl of horse radish peroxidase (HRP) was added followed by the addition of 50 μl of chromogen A and B substrates. The final reaction was read at 450 nm. The cut off values (C.O) for the wells (samples) were calculated according to the manufacturer’s directions using the formula: C. O = Nc + 0.16 (Nc = the mean absorbance value for three negative controls).
Molecular testing for HEV RNA in humans and pigs
Serum viral RNA was extracted from 140 μl of human and animal serum using the spin protocol of QIAamp Viral RNA Mini Kit following the manufacturer’s instructions (Qiagen, Hilden-Germany). HEV RNA detection based on a OneStep Real-Time PCR amplification was performed with reagents from Invitrogen (Thermofisher scientific, USA). The PCR testing was done by preparing a 25 μl reaction volume. The reaction volume was made up of 4.1 μl of RNase free water, 12.5 μl of 2X reaction mix, 0.4 μl of 50 mM MgSO4, 1.0 μl each of forward primer (5′–GGTGGTTTCTGGGGTGAC–3′) and reverse primer (5′–AGGGGTTGGTTGGRTGRA–3′), 0.5 μl of probe (5′–FAM-TGATTCTCAGCC CTTCGC–BHQ1–3′), 0.5 μl of an Invitrogen superscript III One-step RT-PCR Taq enzyme mix and 5.0 μl of the template RNA. In-house in vitro transcript of HEV RNA and RNase free water were included in each run as positive and negative controls respectively.
Master mix preparation and RNA addition were aseptically done in separate rooms to minimise chances of contamination. Cycling conditions for the reaction were; initial reverse transcription at 50 °C for 30 min, polymerase enzyme activation at 94 °C for 3 min, followed by a denaturation step at 94 °C for 15 s. Annealing was achieved at 60 °C for 30 s. The denaturation and annealing steps were repeated for 45 cycles. Acquisition was done after each annealing step. Bio-Rad CFX96 C1000 thermal cycler (Bio-Rad Laboratories Inc., Singapore) was used for the cycling and analyses of the PCR results. According to internal validation, samples were considered negative if the threshold cycle value was > 38, uncertain if between 36 and 38 and positive if less or equal to 36 .
Sequencing and phylogenetic classification of derived sequences
Samples that were positive by real-time RT-PCR were further screened with a hemi-nested RT PCR assay targeting the RNA-dependent RNA polymerase (RdRp) region. This was done to generate amplicons of approximately 338 base pairs as previously described  for confirmation by sequencing. Amplicons were Sanger-sequenced (Microsynth Seqlab, Göttingen, Germany) and overlapping bi-directional sequences were joined and MAFFT sequence alignments done in Geneious R11 (https://www.geneious.com). Classification of derived sequences was performed by phylogenetic comparisons with reference sequences available in GenBank by Bayesian inference using the MrBayes plugin in geneiosus . A general time reversible model with gamma distribution and proportion of invariable sites (GTR + G + I) was used.
Descriptive analysis was performed to determine the frequency and percentages of categorical variables. HEV RNA prevalence with its 95% confidence interval was computed at the animal level and farm based on a binomial distribution assumption. The association between HEV prevalence and sociodemographic or other clinical variables were tested using Fisher’s exact test or Chi-square test where appropriate. Variables which were significant were entered into a logistic regression model to determine the independent risk factors associated with HEV positivity in the pigs. Estimates from the model were expressed as odds ratio (OR) and Wald’s 95% confidence interval (CI). All statistical tests were two-tailed and p ≤ 0.05 were considered statistically significant. Statistical analysis was done using R statistical software (version 3.5.1, 2018).
Categorical variables were expressed as frequencies and percentages for antibodies to HEV detected. Continuous variables were expressed as mean or medians based on the distribution of the data. The association between HEV detection and contact with pigs together with other variables was analysed using Fisher’s exact test or Chi-square test where appropriate. Variables that were significant were entered into a logistic regression model. Confounding variables were monitored by evaluating the change in the coefficient of a factor after removing another factor. Estimates were expressed as OR and 95% Wald’s CI. A statistically significant result was considered when p-value ≤0.05.
Sociodemographic characteristics of study participants
Our study enrolled a total of 544 study participants. The median age of participants was 35 (IQR = 25–50) with more males (303, 56.3%) than females (235; 43.5%). Majority of the participants were from the Upper East region (313; 57.5%). Two hundred and sixty-four (264; 48.5%) were in direct contact with pigs and 280 (51.5%) were not. Most of the subjects were married (374; 69.6%) and many had no formal school education (251; 46.1%).
Hepatitis E testing in humans
All human samples were negative for hepatitis E viral RNA. Two hundred and ten participants were positive for IgG with an overall prevalence of 38.6%. A comparison of HEV antibody positivity for other risk factor variables showed significant differences for age, educational level, religion, marital status, region of participants, the type of drinking water (pipe borne water, borehole) and contact with pigs (Table 1). The frequency of HEV antibodies in contact groups (114; 54.3%) was higher than those in the non-contact groups (96; 45.7%) and the difference was statistically significant in univariate analysis (p = 0.041). However, adjusting for other significant variables in a multivariate logistic regression analysis left no significant difference for HEV prevalence among contact and non-contact groups. Similarly, a higher number of christians were hepatitis E antibody positive as compared to muslims and traditionalists. Although the difference was siginificant in the univariate analysis, further adjustment with other variables at the multivariate level did not show any significance. A further analysis at the multivariate level did not show significant difference for the types of water, education, religion and marital status. The region and age group were however significant risk factors of HEV positivity (Table 2).
Seven hundred and twenty (720) pigs were sampled from 18 farms located in five regions. Of these, 439 (61.0%) were females. Most of the pigs sampled were adults 510 (70.8%) as compared to weaners 210 (29.2%). Based on records of rectal temperature taken, 175 (24.3%) of the pigs had fever and 545 (75.7%) had no fever.
HEV RNA detection in pigs
Twenty-three (23) of the 720 pigs (3.2, 95%CI = 2.0–4.8) were positive for HEV RNA by real-time RT-PCR. The highest number of HEV was detected on farms where most weaners were sampled. The detection rate in weaners (19/210, 9, 95%CI = 5.5–13.8%) was about ten times that in adult animals (4/510, 0.8, 95%CI = 0.2–2.0), which was significantly different (p < 0.001). Detection rates were similar in male vs. female animals (p = 0.126). A multivariate logistic regression showed weaners have 11.57 (95% CI = 3.83,34.94) odds of exposure to HEV compared to adults and this was statistically significant (p < 0.001) as shown in Table 3.
Classification of HEV sequences
All 23 real-time RT-PCR-positive samples were also positive by hemi-nested RT PCR. The sequences obtained form this study were most similar to each other with a maximum sequence identity of 99.84% and a minimum of 87.62%. The most closely related sequence to the ones from this study was another sequence from Ghana (Accession number: MN714358)  with 97.43% as the highest sequence identity followed by a sequence from Germany (Accession number: FJ998008) with a sequence identity of 88.75%. The sequences from this study clustered together with other genotype 3 sequences when compared with other HEV reference sequences  as seen in Fig. 2. All sequences obtained in this study were submited to GenBank and assigned accession numbers MW331262-MW331276.
HEV is considered to be of significant public health importance in many developing countries and likely to thrive in resource-poor regions in Africa and Southeast Asia. The prevalence of anti-HEV has been extensively reviewed in Africa with rates varying from 0% in the general population  to 84.3% in pregnant women . We recently reported a prevalence of 32.9% among jaundiced patients in Ghana .
Although high sero-prevalence has been reported, the source of these infections in resource-poor settings is still under investigation. Two studies conducted in the capital city of Ghana (Accra) reported total IgG prevalence of 34.8 and 38.1% among pig handlers [5, 57]. It was however not clear whether this was associated with pig farminig or common to the general population. We therefore conducted a comparative cross-sectional study to find out whether rearing or staying in close proximity to pigs is associated with exposure to HEV and to further evaluate the risk of zoonotic HEV infection in both swine and humans.
Our study identified a 3.2% prevalence of HEV RNA viremia among pig samples in five regions in Ghana with most cases from farms having a high proportion of weaners. This indicates high transmission and incidence rates during early life when maternal immunity begins to wane, which corresponds to findings from other regions with intensive hog farming . Also, the prevalence of viremia was similar to studies of HEV in Europe . All HEV RNA samples were identified as genotype 3, suggesting a risk of zoonotic hepatitis E like in other regions of the world . Our search for swine-related factors associated with human infection therefore seems justified.
Several reports in industrialised countries support a link between presence of anti-HEV and direct contact with pigs. Studies in United States , Switzerland , Germany , Australia  found significant differences in prevalence of individuals in close contact with pigs compared to those not exposed. On the contrary other studies conducted in Sweden  and Brazil  found no significant differences between those exposed or unexposed to pigs. Data are more limited in Africa. Previous studies in Ghana and Madagascar investigated only pig contacts but no simultaneous control groups [5, 66]. One study in Uganda identified high HEV antibodies among pig contact groups as compared to non-contact groups .
Whereas we have not been able to detect viral RNA in any of our human subjects and thereby failed to prove transmission by sequence identity, the design of our study permits some relevant conclusions based on seroprevalence. For instance, the risk of HEV infection was independently associated with age. People aged more than 32 years had the highest exposure as compared to young groups (< 32). Knowing that antibodies against HEV persist for prolonged time , seroprevalence can only summarize cumulative exposures per group rather than being able to indicate recent infection  (it should be mentioned, however, that some authors consider anti-HEV antibodies to be short-lived and wane after ca 5 years ). As seroprevalence was high also in the group without direct contact, this can explain why we have not seen a correlation between direct pig contact and seropositivity. Transmission events in our study are likely to be reflected by serology on a cumulative basis, and also are assumed to include indirect exposures such as via fomites or water, as indicated by increased seroprevalence in parts of the population with lower levels of education, less favourable sanitary conditions, and no access to tap water. These are among the expected patterns of seroprevalence also for HEV genotypes 1 and 2, and these genotypes may account for a part of the seroprevalence observed in humans. Other examples such as the different seroprevalence rates between Christian and Muslim groups, however, point toward acquisition of HEV based on pork consumption. One limitation of this study was our inability to collect information on duration for contact with pigs or interaction in pig environments. Collection of this information would have improved further risk factor analysis in multivariate logistics regression. Further studies could consider inclusion of such variables in order to determine the levels of risk in contact and non-contact groups.
Our data confirm previous reports of high endemicity of HEV in Ghana. Active surveillance and introduction of vaccines could be helpful to reducing active infections in human. We have also provided evidence that pigs in Ghana have infectious form of HEV circulating among them and this could pose significant risk to zoonotic infection in human.
Availability of data and materials
Data generated or analysed during this study are included in this published article. Sequences are available via NCBI GenBank. Further supporting data if required are available from the corresponding author on reasonable request.
Committee for Human Research, Publications and Ethics
Enzyme-Linked Immunosorbent Assay
- GTR + G + I:
General time reversible model with a gamma distribution and proportion of invariable sites
Hepatitis E virus
Horse radish peroxidase
Komfo Anokye Teaching Hospital
Kumasi Centre for Collaborative Research into Tropical Medicine
Kwame Nkrumah University of Science and Technology
Multiple Alignment using Fast Fourier Transform
Mean absorbance value for three negative controls
Polymerase Chain Reaction
RNA-dependent RNA polymerase
United States of America
Van der Poel W, Verschoor F, van der Heide R, Herrera M-I, Vivo A, Kooreman M, et al. Hepatitis E virus sequences in swine related to sequences in humans, The Netherlands. Emerg Infect Dis. 2001;7(6):970–6. https://doi.org/10.3201/eid0706.010608.
Purcell RH, Emerson SU. Hidden danger: the raw facts about hepatitis E virus. J Infect Dis. 2010;202(6):819–21. https://doi.org/10.1086/655900.
Pauli G, Aepfelbacher M, Bauerfeind U, Blümel J, Burger R, Gärtner B, et al. Hepatitis E virus. Transfus Med Hemother. 2015;42(4):247–65. https://doi.org/10.1159/000431191.
Galiana C, Fernández-Barredo S, García A, Gómez MT, Pérez-Gracia MT. Occupational exposure to hepatitis E virus (HEV) in swine workers. Am J Trop Med Hyg. 2008;78(6):1012–5. https://doi.org/10.4269/ajtmh.2008.78.1012.
Adjei A, Aviyase J, Tettey Y, Adu-Gyamfi C, Mingle J, Ayeh-Kumi P, et al. Heatitis E virus infection among pig handlers in Accra, Ghana. East Afr Med J. 2009;86(8):359–63.
Balayan MS, Andjaparidze AG, Savinskaya SS, Ketiladze ES, Braginsky DM, Savinov AP, et al. Evidence for a virus in non-A, non-B hepatitis transmitted via the fecal-oral route. Intervirology. 1983;20(1):23–31. https://doi.org/10.1159/000149370.
Vasickova P, Psikal I, Kralik P, Widen F, Hubalek Z, Pavlik I. Hepatitis E virus: a review. Vet Med Praha. 2007;52(9):365.
Lozano R, Naghavi M, Foreman K, Lim S, Shibuya K, Aboyans V, et al. Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the global burden of disease study 2010. Lancet. 2012;380(9859):2095–128.
Tsega E, Krawczynski K, Hansson B, Nordenfelt E. Hepatitis E virus infection in pregnancy in Ethiopia. Ethiop Med J. 1993;31(3):173–81.
Pérez-Gracia MT, García M, Suay B, Mateos-Lindemann ML. Current knowledge on hepatitis E. J Clin Transl Hepatol. 2015;3(2):117–26. https://doi.org/10.14218/JCTH.2015.00009.
Ankcorn M, Tedder R. Hepatitis E: the current state of play. Transfus Med. 2017;27(2):84–95. https://doi.org/10.1111/tme.12405.
Tei S, Kitajima N, Takahashi K, Mishiro S. Zoonotic transmission of hepatitis E virus from deer to human beings. Lancet. 2003;362(9381):371–3. https://doi.org/10.1016/S0140-6736(03)14025-1.
Di Bartolo I, Angeloni G, Ponterio E, Ostanello F, Ruggeri FM. Detection of hepatitis E virus in pork liver sausages. Int J Food Microbiol. 2015;193:29–33. https://doi.org/10.1016/j.ijfoodmicro.2014.10.005.
Dalton HR, van Eijk JJ, Cintas P, Madden RG, Jones C, Webb GW, et al. Hepatitis E virus infection and acute non-traumatic neurological injury: a prospective multicentre study. J Hepatol. 2017;67(5):925–32. https://doi.org/10.1016/j.jhep.2017.07.010.
Kamar N, Bendall RP, Peron JM, Cintas P, Prudhomme L, Mansuy JM, et al. Hepatitis E virus and neurologic disorders. Emerg Infect Dis. 2011;17(2):173–9. https://doi.org/10.3201/eid1702.100856.
Shao Z-j, Li J-h, Zheng Y-j, Zhang J-x, Ma Y-h, Ma W-t, et al. Epidemiological screening for hepatitis E virus in bile specimens from livestock in Northwest China. J Clin Microbiol. 2009;47(3):814–6.
Meng X-J, Halbur PG, Shapiro MS, Govindarajan S, Bruna JD, Mushahwar IK, et al. Genetic and experimental evidence for cross-species infection by swine hepatitis E virus. J Virol. 1998;72(12):9714–21. https://doi.org/10.1128/JVI.72.12.9714-9721.1998.
Halbur P, Kasorndorkbua C, Gilbert C, Guenette D, Potters M, Purcell R, et al. Comparative pathogenesis of infection of pigs with hepatitis E viruses recovered from a pig and a human. J Clin Microbiol. 2001;39(3):918–23. https://doi.org/10.1128/JCM.39.3.918-923.2001.
Yugo DM, Meng X-J. Hepatitis E virus: foodborne, waterborne and zoonotic transmission. Int J Environ Res Public Health. 2013;10(10):4507–33. https://doi.org/10.3390/ijerph10104507.
Wang B, Harms D, Papp CP, Niendorf S, Jacobsen S, Lütgehetmann M, et al. Comprehensive molecular approach for characterization of hepatitis e virus genotype 3 variants. J Clin Microbiol. 2018. https://doi.org/10.1128/JCM.01686-17.
Zhang Y, Wang L, Zeng H, Gong W, Wang L, Zhuang H. Development and validation of a new serum standard for the measurement of anti-HEV antibodies in animals. J Med Virol. 2017;89(3):497–501. https://doi.org/10.1002/jmv.24651.
Van der Poel W, Pavio N, Van der Goot J, Van Es M, Martin M, Engel B. Development and validation of a genotype 3 recombinant protein-based immunoassay for hepatitis E virus serology in swine. Braz J Med Biol Res. 2014;47(4):334–9. https://doi.org/10.1590/1414-431X20133249.
Krumbholz A, Joel S, Neubert A, Dremsek P, Dürrwald R, Johne R, et al. Age-related and regional differences in the prevalence of hepatitis E virus-specific antibodies in pigs in Germany. Vet Microbiol. 2013;167(3–4):394–402. https://doi.org/10.1016/j.vetmic.2013.10.001.
Ijaz S, Arnold E, Banks M, Bendall RP, Cramp ME, Cunningham R, et al. Non–travel-associated hepatitis E in England and wales: demographic, clinical, and molecular epidemiological characteristics. J Infect Dis. 2005;192(7):1166–72. https://doi.org/10.1086/444396.
Dalton HR, Stableforth W, Thurairajah P, Hazeldine S, Remnarace R, Usama W, et al. Autochthonous hepatitis E in Southwest England: natural history, complications and seasonal variation, and hepatitis E virus IgG seroprevalence in blood donors, the elderly and patients with chronic liver disease. Eur J Gastroenterol Hepatol. 2008;20(8):784–90. https://doi.org/10.1097/MEG.0b013e3282f5195a.
Mansuy JM, Peron JM, Abravanel F, Poirson H, Dubois M, Miedouge M, et al. Hepatitis E in the south west of France in individuals who have never visited an endemic area. J Med Virol. 2004;74(3):419–24. https://doi.org/10.1002/jmv.20206.
Mitsui T, Tsukamoto Y, Hirose A, Suzuki S, Yamazaki C, Masuko K, et al. Distinct changing profiles of hepatitis A and E virus infection among patients with acute hepatitis, patients on maintenance hemodialysis and healthy individuals in Japan. J Med Virol. 2006;78(8):1015–24. https://doi.org/10.1002/jmv.20657.
Mansuy JM, Legrand-Abravanel F, Calot JP, Peron JM, Alric L, Agudo S, et al. High prevalence of anti-hepatitis E virus antibodies in blood donors from south West France. J Med Virol. 2008;80(2):289–93. https://doi.org/10.1002/jmv.21056.
Hewitt J, Harte D, Sutherland M, Croucher D, Fouche L, Flanagan P, et al. Prevalence of hepatitis E virus antibodies and infection in New Zealand blood donors. N Z Med J. 2018;131(1469):38–43.
Adlhoch C, Avellon A, Baylis SA, Ciccaglione AR, Couturier E, de Sousa R, et al. Hepatitis E virus: assessment of the epidemiological situation in humans in Europe, 2014/15. J Clin Virol. 2016;82:9–16. https://doi.org/10.1016/j.jcv.2016.06.010.
Adjei AA, Aviyase JT, Tettey Y, Adu-Gyamfi C, Mingle JA, Ayeh-Kumi PF, et al. Hepatitis E virus infection among pig handlers in Accra, Ghana. East Afr Med J. 2009;86(8):359–63.
Adjei AA, Tettey Y, Aviyase JT, Adu-Gyamfi C, Mingle JA, Nartey ET. Unexpected elevated alanine aminotransferase, aspartate aminotransferase levels and hepatitis E virus infection among persons who work with pigs in Accra, Ghana. Virol J. 2010;7(1):1–9.
Adjei AA, Tettey Y, Aviyase JT, Adu-Gyamfi C, Obed S, Mingle JA, et al. Hepatitis E virus infection is highly prevalent among pregnant women in Accra, Ghana. Virol J. 2009;6(1):1–5.
Tettey Y, Adjei A, Ayivase J, Adu-Gyamfi C, Mingle J, Nartey E, et al. Serological evidence of hepatitis E virus infection among volunteer blood donors at the Accra area blood transfusion center, Accra, Ghana. J Ghana Sci Assoc. 2011;13(2):64–73.
Martinson FE, Marfo VY, Degraaf J. Hepatitis E virus seroprevalence in children living in rural Ghana. West Afr J Med. 1999;18(2):76–9.
Feldt T, Sarfo FS, Zoufaly A, Phillips RO, Burchard G, van Lunzen J, et al. Hepatitis E virus infections in HIV-infected patients in Ghana and Cameroon. J Clin Virol. 2013;58(1):18–23. https://doi.org/10.1016/j.jcv.2013.05.004.
Ofosu-Appiah L, Boney J, Adiku T, Barnor J, Odoom J. Serological and molecular evidence of hepatitis E infection among patients with suspected viral hepatitis seen at Korle-Bu teaching hospital, Accra. J Sci Res Rep. 2016;10(1):1–10.
Meldal B, Sarkodie F, Owusu-Ofori S, Allain JP. Hepatitis E virus infection in Ghanaian blood donors–the importance of immunoassay selection and confirmation. Vox Sang. 2013;104(1):30–6. https://doi.org/10.1111/j.1423-0410.2012.01637.x.
Bonney JHK, Osei-Kwasi M, Adiku TK, Barnor JS, Amesiya R, Kubio C, et al. Hospital-based surveillance for viral hemorrhagic fevers and hepatitides in Ghana. PLoS Negl Trop Dis. 2013;7(9):e2435. https://doi.org/10.1371/journal.pntd.0002435.
Bonney JHK, Kwame-Aryee RA, Obed S, Tamatey AA, Barnor JS, Armah NB, et al. Fatal hepatitis E viral infection in pregnant women in Ghana: a case series. BMC Res Notes. 2012;5(1):478.
Shirazi R, Pozzi P, Wax M, Bar-Or I, Asulin E, Lustig Y, et al. Hepatitis E in pigs in Israel: seroprevalence, molecular characterisation and potential impact on humans. Euro Surveill. 2018;23(49):1800067.
Cannon R, Roe R. Livestock disease surveys: a field manualforveterinarians. Canberra: Australia Australian Gvt Publishing Service; 1982.
Zhang Z, Zhang H, Liu T. Study on body temperature detection of pig based on infrared technology: a review. Artif Intell Agric. 2019;1:14–26. https://doi.org/10.1016/j.aiia.2019.02.002.
Olsen B, Axelsson-Olsson D, Thelin A, Weiland O. Unexpected high prevalence of IgG-antibodies to hepatitis E virus in Swedish pig farmers and controls. Scand J Infect Dis. 2006;38(1):55–8. https://doi.org/10.1080/00365540500321470.
Wenzel JJ, Preiss J, Schemmerer M, Huber B, Jilg W. Test performance characteristics of anti-HEV IgG assays strongly influence hepatitis E seroprevalence estimates. J Infect Dis. 2012;207(3):497–500. https://doi.org/10.1093/infdis/jis688.
Yan Q, Du H-l, Wang Y-b, Ge S-x, Huang S-j, Jiang L, et al. Comparison of two diagnostic reagents to detect anti-hepatitis E virus IgG antibodies. Chin J Zoonoses. 2008;24:1087–9.
Capai L, Falchi A, Charrel R. Meta-analysis of human IgG anti-HEV seroprevalence in industrialized countries and a review of literature. Viruses. 2019;11(1):84. https://doi.org/10.3390/v11010084.
Bohm K, Strömpl J, Krumbholz A, Zell R, Krause G, Sievers C. Establishment of a highly sensitive assay for detection of hepatitis E virus-specific immunoglobulins. J Clin Microbiol. 2020;58(2):e01029–19.
Caruso C, Modesto P, Bertolini S, Peletto S, Acutis P, Dondo A, et al. Serological and virological survey of hepatitis E virus in wild boar populations in northwestern Italy: detection of HEV subtypes 3e and 3f. Arch Virol. 2015;160(1):153–60. https://doi.org/10.1007/s00705-014-2246-5.
Drexler JF, Seelen A, Corman VM, Fumie Tateno A, Cottontail V, Melim Zerbinati R, et al. Bats worldwide carry hepatitis E virus-related viruses that form a putative novel genus within the family Hepeviridae. J Virol. 2012;86(17):9134–47. https://doi.org/10.1128/JVI.00800-12.
Huelsenbeck JP, Ronquist F. MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics. 2001;17(8):754–5. https://doi.org/10.1093/bioinformatics/17.8.754.
El-Duah P, Dei D, Binger T, Sylverken A, Wollny R, Tasiame W, et al. Detection and genomic characterization of hepatitis E virus genotype 3 from pigs in Ghana, Africa. One Health Outlook. 2020;2(1):1–9.
Smith DB, Simmonds P, Izopet J, Oliveira-Filho EF, Ulrich RG, Johne R, et al. Proposed reference sequences for hepatitis E virus subtypes. J Gen Virol. 2016;97(3):537–42. https://doi.org/10.1099/jgv.0.000393.
El-Esnawy N. Examination for hepatitis E virus in wastewater treatment plants and workers by nested RT-PCR and ELISA. J Egypt Public Health Assoc. 2000;75(1–2):219–31.
Stoszek SK, Abdel-Hamid M, Saleh DAA, Kafrawy SE, Narooz S, Hawash Y, et al. High prevalence of hepatitis E antibodies in pregnant Egyptian women. Trans R Soc Trop Med Hyg. 2006;100(2):95–101. https://doi.org/10.1016/j.trstmh.2004.12.005.
Owusu M, Bonney JK, Annan AA, Mawuli G, Okyere K, Mutocheluh M, et al. Aetiology of viral hepatitis among jaundiced patients presenting to a tertiary hospital in Ghana. PLoS One. 2018;13(9):e0203699. https://doi.org/10.1371/journal.pone.0203699.
Adjei AA, Tettey Y, Aviyase JT, Adu-Gyamfi C, Mingle JA, Nartey ET. Unexpected elevated alanine aminotransferase, asparte aminotransferase levels and hepatitis E virus infection among persons who work with pigs in Accra, Ghana. Virol J. 2010;7(1):336. https://doi.org/10.1186/1743-422X-7-336.
Meng XJ. Hepatitis E virus: animal reservoirs and zoonotic risk. Vet Microbiol. 2010;140(3–4):256–65. https://doi.org/10.1016/j.vetmic.2009.03.017.
Christensen PB, Engle RE, Hjort C, Homburg KM, Vach W, Georgsen J, et al. Time trend of the prevalence of hepatitis E antibodies among farmers and blood donors: a potential zoonosis in Denmark. Clin Infect Dis. 2008;47(8):1026–31. https://doi.org/10.1086/591970.
Pavio N, Doceul V, Bagdassarian E, Johne R. Recent knowledge on hepatitis E virus in Suidae reservoirs and transmission routes to human. Vet Res. 2017;48(1):78. https://doi.org/10.1186/s13567-017-0483-9.
Meng X, Wiseman B, Elvinger F, Guenette D, Toth T, Engle R, et al. Prevalence of antibodies to hepatitis E virus in veterinarians working with swine and in normal blood donors in the United States and other countries. J Clin Microbiol. 2002;40(1):117–22. https://doi.org/10.1128/JCM.40.1.117-122.2002.
Kaufmann A, Kenfak-Foguena A, Andre C, Canellini G, Bürgisser P, Moradpour D, et al. Hepatitis E virus seroprevalence among blood donors in Southwest Switzerland. PLoS One. 2011;6(6):e21150. https://doi.org/10.1371/journal.pone.0021150.
Krumbholz A, Mohn U, Lange J, Motz M, Wenzel JJ, Jilg W, et al. Prevalence of hepatitis E virus-specific antibodies in humans with occupational exposure to pigs. Med Microbiol Immunol. 2012;201(2):239–44. https://doi.org/10.1007/s00430-011-0210-5.
Taus K, Schmoll F, El-Khatib Z, Auer H, Holzmann H, Aberle S, et al. Occupational swine exposure and hepatitis E virus, Leptospira, Ascaris suum seropositivity and MRSA colonization in Austrian veterinarians, 2017–2018—a cross-sectional study. Zoonoses Public Health. 2019;66(7):842–51. https://doi.org/10.1111/zph.12633.
Silva SMT, Oliveira JM, Vitral CL, Vieira KA, Pinto MA, Souto FJD. Prevalence of hepatitis E virus antibodies in individuals exposed to swine in Mato Grosso, Brazil. Mem Inst Oswaldo Cruz. 2012;107(3):338–41. https://doi.org/10.1590/S0074-02762012000300007.
Temmam S, Besnard L, Andriamandimby SF, Foray C, Rasamoelina-Andriamanivo H, Héraud J-M, et al. High prevalence of hepatitis E in humans and pigs and evidence of genotype-3 virus in swine, Madagascar. Am J Trop Med Hyg. 2013;88(2):329–38. https://doi.org/10.4269/ajtmh.2012.12-0615.
Ukuli AQ, Mugimba KK. Seroprevalence of hepatitis E in swine abattoir workers. Afr Health Sci. 2017;17(4):1022–8. https://doi.org/10.4314/ahs.v17i4.9.
Corwin A, Putri M, Winarno J, Lubis I, Suparmanto S, Sumardiati A, et al. Epidemic and sporadic hepatitis E virus transmission in West Kalimantan (Borneo), Indonesia. Am J Trop Med Hyg. 1997;57(1):62–5. https://doi.org/10.4269/ajtmh.1997.57.62.
Arankalle VA, Tsarev SA, Chadha MS, Alling DW, Emerson SU, Banerjee K, et al. Age-specific prevalence of antibodies to hepatitis A and E viruses in Pune, India, 1982 and 1992. J Infect Dis. 1995;171(2):447–50. https://doi.org/10.1093/infdis/171.2.447.
Rapicetta M, Kondili LA, Pretolani S, Stroffolini T, Chionne P, Villano U, et al. Seroprevalence and anti-HEV persistence in the general population of the Republic of San Marino. J Med Virol. 1999;58(1):49–53. https://doi.org/10.1002/(SICI)1096-9071(199905)58:1<49::AID-JMV7>3.0.CO;2-C.
This research was funded by Deutsche Forschungsgemeinschaft (DR 772/12–1). Open Access funding enabled and organized by Projekt DEAL.
Ethics approval and consent to participate
The study protocol was approved by the Committee for Human Research, Publications and Ethics (CHRPE) of the Komfo Anokye Teaching Hospital (KATH) and School of Medical Sciences, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ashanti region, Ghana (Approval number, AP/167/17). Permission for livestock sampling was also obtained from the wildlife division of the Ghana Forestry Commission (Approval Number: AO4957).
Consent for publication
Informed consent was obtained from all subjects involved in the study.
Christian Drosten is an Editorial Board Member for One Health Outlook. The authors declare that they have no other competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Yeboah, R., Sylverken, A.A., Owusu, M. et al. Sero-molecular epidemiology of hepatitis E virus in pigs and human contacts in Ghana. One Health Outlook 3, 13 (2021). https://doi.org/10.1186/s42522-021-00043-w
- One health
- Infectious disease reservoirs
- Viral hepatitis