Table 5 Distribution of substitutions in topoisomerase subunits detected in 43 PMQR Escherichia coli isolates from humans, chickens and poultry farm/market environments
Target Enzymes | Mutation | Nucleotide change | Amino acid substitution | n = 43 (%) |
|---|---|---|---|---|
DNA gyrase gyrA | S83L; D87N | TCG → TTG; GAC → AAC | S → L; D → N | 20 (46.5) |
S83L | TCG → TTG | S → L | 18 (41.9) | |
S83L; D87Y | TCG → TTG; GAC → TAC | S → L; D → Y | 1 (2.3) | |
S83A | TCG → GCG | S → A | 1 (2.3) | |
Topoisomerase IV parC | S80I | AGC → ATC | S → I | 22 (51.2) |
S80I; A56T | AGC → ATC; GCC → ACC | S → I; A → T | 2 (4.7) | |
S80I; E84G | AGC → ATC; GAA → GGA | S → I; E → G | 1 (2.3) | |
S80I; E84G; A56T | AGC → ATC; GAA → GGA; GCC → ACC | S → I; E → G; A → T | 1 (2.3) | |
A56T | GCC → ACC | A → T | 3 (7.0) | |
S57T | AGC → ACC | S → T | 1 (2.3) | |
parE | S458A | TCG → GCG | S → A | 6 (14.0) |
L416F | CTT → TTT | L → F | 3 (7.0) | |
I355T | ATC → ACC | I → T | 1 (2.3) | |
pmrB | V161G | GTG → GGG | V → G | 1 (2.3) |