Table 1 PCR primers for diarrhoeagenic Escherichia coli and 16srRNA gene
Type | Primer Designation | Primers (5 to 3) | Target gene | Amplicon size (bp) |
|---|---|---|---|---|
ECO | ECO-1 | GACCTCGGTTTAGTTCACAGA | 16SrRNA | 585 |
ECO-2 | CACACGCTGACGCTGACCA | |||
EPEC | eae 1 | CTGAACGGCGATTACGCGAA | eae | 917 |
eae 2 | CCAGACGATACGATCCAG | |||
bfp 1 | AATGGGCTTGCGCTTCCAG | bfpA | 326 | |
bfp 2 | GCCGCTTTATCCAACCTGGTA | |||
EAEC | EAEC1 | CTGGCGAAAGACTGTATCAT | CVD432 | 630 |
EAEC2 | CAATGTATAGAAATCCGCTGTT | |||
ETEC | LTf | GGCGACAGATTATACCGTGC | LT | 450 |
LTr | CAATGTATAGAAATCCGCTGTT | |||
STf | ATTTTTMTTTCTGTATTRTCTT | ST | 190 | |
STr | CACCCGGTACARGCAGGATT | |||
EIEC | IpaH1 | GTTCCTTGACCGCCTTTCCGATACCGTC | ipaH | 600 |
IpaH2 | GCCGGTCAGCCACCCTCTGAGAGTAC | |||
EHEC | Stx1f | ATAAATCGCCATTCGTTGACTAC | Stx1 | 180 |
Stx1r | AGAACGCCCACTGAGATCATCC | |||
Stx2f | GGCACTGTCTGAAACTGCTCC | Stx2 | 255 | |
Stx2r | TCGCCAGTTATCTGACATTCTG |