Table 1 Indirect ELISA to compare reactivity of recombinant GP proteins from EBOV, SUDV, RESTV, BDBV, TAFV, BOMV and MARV with polyclonal antibodies against all seven viruses (++++ = O.D. > 4.0, +++ = O.D. 3.0–4.0, ++ = O.D. 2.0–3.0, + = O.D. > 0.5–2.0). - = non-reactive. As expected, some cross-reactivity does occur but differential detection of antibodies against a specific virus is possible
| GP Antigen | |||||||
|---|---|---|---|---|---|---|---|
| Antibody | EBOV | SUDV | RESTV | BDBV | TAFV | BOMV | MARV |
| Anti-EBOV | ++++ | + | ++ | ++ | ++ | + | + |
| Anti-SUDV | – | +++ | – | – | – | – | – |
| Anti-RESTV | – | – | ++++ | – | – | – | – |
| Anti-BDBV | + | – | + | ++++ | + | – | – |
| Anti-TAFV | – | – | – | + | +++ | – | – |
| Anti-BOMV | – | – | – | – | + | ++++ | – |
| Anti-MARV | – | – | – | – | – | – | +++ |